Anti-SEMA4D / CD100 Reference Antibody (pepinemab)

• Catalog Number: orb1818313
• Category: Antibodies
• Description: Recombinant Antibody
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human, Mouse
• Isotype: IgG4SP
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Form/Appearance: Purified monoclonal antibody supplied in PBS, pH 6.0, without preservative.This antibody is purified through a protein A column.
• Conjugation: Unconjugated
• MW: 145.88
• UniProt ID: Q92854
• Protein Sequence: SEMA4D / CD100
• Expression System: CHO Cell
• Endotoxins: < 0.001EU/μg,determined by LAL method.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Semaphorin-4D, A8, BB18, GR3, CD100, SEMA4D, C9orf
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The purity of Anti-SEMA4D / CD100 Reference Antibody (pepinemab) is more than 100%, determined by SEC-HPLC.

Immobilized human SEMA4D/CD100, His Tag at 2 µg/mL can bind Anti-SEMA4D / CD100 Reference Antibody (pepinemab), EC50=0.0081 µg/mL

Pepinemab inhibited the tumor growth of CT26 on balb/c mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 76.9% at 50 mpk at D24.

Anti-SEMA4D / CD100 Reference Antibody (pepinemab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%

Human C100 HEK293 cells were stained with Anti-SEMA4D / CD100 Reference Antibody (pepinemab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC231=0.3227 nM