SECTM1/Sectm1b Rabbit Polyclonal Antibody

SKU: orb315187

Description

Anti-SECTM1/Sectm1b Antibody. Tested in WB applications. This antibody reacts with Mouse.

Research Area

Cancer Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Mouse
• Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse SECTM1.
• Target: Secreted and transmembrane protein 1b
• Molecular Weight: 23 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Secreted and transmembrane protein 1b; Protein K-12; Sectm1b; K12, Sectm1

Western blot analysis of SECTM1 using anti-SECTM1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HEPA Whole Cell Lysate at 40 ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SECTM1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SECTM1 at approximately 23 kDa. The expected band size for SECTM1 is at 23 kDa.

Quick Database Links

Gene Symbol

Secreted and transmembrane protein 1b

UniProt

Q9JL59