Scramblase 1/PLSCR1 Antibody

SKU: orb623817

Description

Anti-Scramblase 1/PLSCR1 Antibody. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Application Notes:
  Western blot, 0.1-0.25μg/ml, Human, Mouse Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse ELISA, 0.1-0.5μg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Scramblase 1/PLSCR1 recombinant protein (Position: M1-S54).
• Molecular Weight: 35 kDa
• Purification: Immunogen affinity purified.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Disclaimer: For research use only

Alternative Names

Phospholipid scramblase 1; PL scramblase 1; Ca (2+)-dependent phospholipid scramblase 1; Erythrocyte phospholipid scramblase; MmTRA1b; PLSCR1

IHC analysis of PLSCR1 using anti-PLSCR1 antibody. PLSCR1 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-PLSCR1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Western blot analysis of PLSCR1 using anti-PLSCR1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLSCR1 antigen affinity purified polyclonal antibody at 0.25 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PLSCR1 at approximately 35 kDa. The expected band size for PLSCR1 is at 35 kDa.

Quick Database Links

UniProt

O15162