SCN2A Rabbit Polyclonal Antibody

SKU: orb692207

Description

Anti-SCN2A Antibody. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cardiovascular Research, Metabolism Research, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: Western blot, 0.25-0.5μg/ml, Mouse, Rat Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SCN2A, which shares 88.2% amino acid (aa) sequence identity with rat SCN2A.
• Target: Sodium channel protein type 2 subunit alpha
• Molecular Weight: 320 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

sodium voltage-gated channel alpha subunit 2; BFIC3; BFIS3; BFNIS; DEE11; EA9; EIEE11; HBA; HBSCI; HBSCII; NAC2; Na(v)1.2; Nav1.21; SCN2A2; SCN2A

Flow Cytometry analysis of U20S cells using anti-SCN2A antibody. Overlay histogram showing U20S cells (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SCN2A Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Western blot analysis of SCN2A using anti-SCN2A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: rat C6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCN2A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SCN2A at approximately 320 KD. The expected band size for SCN2A is at 320 KD.

Quick Database Links

Gene Symbol

Sodium channel protein type 2 subunit alpha

UniProt

Q99250