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Item 1 of 3
Anti-RXR alpha (Phospho-S260) Antibody
Catalog Number: orb515283
| Catalog Number | orb515283 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to RXRA (phospho-S260) |
| Target | RXRA |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S260 of human RXR alpha protein. The exact sequence is proprietary. |
| UniProt ID | P19793, P28700, Q05343 |
| Tested applications | IH, WB |
| Dilution range | WB: 1:500-1000 |
| Antibody Type | Primary Antibody |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | NR2B1; Retinoic acid receptor RXR-alpha; Nuclear r Read more... |
| Note | For research use only |
| Entrez | 20181, 6256 |
Western blot analysis of RXR alpha (Phospho-S260) expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 53 kD)
Immunohistochemical analysis of RXR alpha (Phospho-S260) staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Direct ELISA antibody dose-response curve using Anti-RXR alpha (Phospho-S260) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
