Anti-RPB5/POLR2E Antibody

• Catalog Number: orb1743957
• Category: Antibodies
• Description: Anti-RPB5/POLR2E Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, WB
• Reactivity: Human, Rat
• Immunogen: E.coli-derived human RPB5/POLR2E recombinant protein (Position: Q95-Q210).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5 μg/ml, Human, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 23 kDa
• UniProt ID: P19388
• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HeLa cells using anti-RPB5/POLR2E antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of RPB5/POLR2E using anti-RPB5/POLR2E antibody.Lane 1:human A431 cell;2:human HeLa cell;3:human HepG2 cell;4:human MCF-7 cell;5:rat liver tissue.

IF analysis of RPB5/POLR2E using anti-RPB5/POLR2E antibody and anti-Beta Tubulin antibody. RPB5/POLR2E was detected in immunocytochemical section of U2OS cell.