Raf1 Rabbit Polyclonal Antibody

SKU: orb402361

Description

The Raf1 Rabbit Polyclonal Antibody is an unconjugated antibody raised in rabbit. It is immunogen affinity purified and suitable for use in FC and WB applications. This antibody targets the RAF proto-oncogene serine/threonine-protein kinase in Human, Mouse, and Rat samples.

Research Area

Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
• Reactivity: Human, Mouse, Rat
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Raf1 recombinant protein (Position: Y364-Q451). Human Raf1 shares 98.9% amino acid (aa) sequence identity with both mouse and rat Raf1.
• Target: RAF proto-oncogene serine/threonine-protein kinase
• Molecular Weight: 73 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RAF proto-oncogene serine/threonine-protein kinase; 2.7.11.1; Proto-oncogene c-RAF; cRaf; Raf-1; RAF1; RAF

Quick Database Links

Gene Symbol

RAF proto-oncogene serine/threonine-protein kinase

UniProt

P04049