Prostate Specific Antigen/KLK3 Rabbit Polyclonal Antibody

SKU: orb738451

Description

Anti-Prostate Specific Antigen/KLK3 Antibody. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Research Area

Epigenetics & Chromatin, Molecular Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: Western blot, 0.25-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human ELISA, 0.1-0.5μg/ml, -
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Prostate Specific Antigen/KLK3 recombinant protein (Position: A64-D255).
• Target: Prostate-specific antigen
• Molecular Weight: 34 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APS; Gamma seminoprotein; hK3; Kallikrein 3; kallikrein related peptidase 3; KLK2A1; KLK3; KLK3/PSA; P 30 antigen; Prostate specific antigen; PSA; Semenogelase; Seminin

IHC analysis of Prostate Specific Antigen/KLK3 using anti-Prostate Specific Antigen/KLK3 antibody. Prostate Specific Antigen/KLK3 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Prostate Specific Antigen/KLK3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Western blot analysis of Prostate Specific Antigen/KLK3 using anti-Prostate Specific Antigen/KLK3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human LNCAP whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prostate Specific Antigen/KLK3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Prostate Specific Antigen/KLK3 at approximately 34 KD. The expected band size for Prostate Specific Antigen/KLK3 is at 34 KD.

Quick Database Links

Gene Symbol

Prostate-specific antigen

UniProt

P07288