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Anti-Prolactin Receptor/PRLR Antibody

Catalog Number: orb334514

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb334514
CategoryAntibodies
DescriptionAnti-Prolactin Receptor/PRLR Antibody. Tested in WB applications. This antibody reacts with Human.
ClonalityPolyclonal
Species/HostRabbit
IsotypeRabbit IgG
ConjugationUnconjugated
ReactivityHuman
Form/AppearanceLyophilized
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
ImmunogenA synthetic peptide corresponding to a sequence at the C-terminus of human PRLR, different from the related mouse sequence by eleven amino acids, and from the related rat sequence by fourteen amino acids.
UniProt IDP16471
MW90 kDa
Tested applicationsWB
Application notesWestern blot, 0.1-0.5μg/ml, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesProlactin receptor; PRL-R; PRLR
NoteFor research use only
Anti-Prolactin Receptor/PRLR Antibody

Western blot analysis of PRLR using anti-PRLR antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRLR antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRLR at approximately 65, 95 kDa. The expected band size for PRLR is at 70 kDa.

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    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
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    IHC,  WB

    Human, Mouse, Rat

    Rabbit

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    IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

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    IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    APC

    100 μg
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    IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    HRP

    100 μg