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Description
Research Area
Signal Transduction
Images & Validation
−| Tested Applications | ELISA, FC, ICC, IF, WB |
|---|---|
| Dilution Range | Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml |
| Reactivity | Human |
| Application Notes |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | E.coli-derived human PRAG1 recombinant protein (Position: H2-V1104). Human PRAG1 shares 74.1% and 73.4% amino acid (aa) sequence identity with mouse and rat PRAG1, respectively. |
| Target | Inactive tyrosine-protein kinase PRAG1 |
| Molecular Weight | 200 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PRAG1; SGK223; Inactive tyrosine-protein kinase PRAG1; PEAK1-related kinase-activating pseudokinase 1; Pragmin; Sugen kinase 223; SgK223
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−PRAG1 Rabbit Polyclonal Antibody (Carrier-free) [orb3091886]
ELISA, FC, ICC, IF, WB
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Rabbit
Polyclonal
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100 μg

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Quick Database Links
Gene Symbol
Inactive tyrosine-protein kinase PRAG1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)



