PPCS Rabbit Polyclonal Antibody

SKU: orb527034

Description

The PPCS Rabbit Polyclonal Antibody is an unconjugated antibody raised in rabbit. It is immunogen affinity purified and suitable for use in FC, ICC, IF, IHC, IHC-Fr, and WB applications. This antibody targets the Phosphopantothenate--cysteine ligase in Human, Mouse, and Rat samples.

Research Area

Metabolism Research, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry (Fixed), 1-3μg/1x10^6 cells
• Reactivity: Human, Mouse, Rat
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PPCS, which shares 73.9% and 78.2% amino acid (aa) sequence identity with mouse and rat PPCS, respectively.
• Target: Phosphopantothenate--cysteine ligase
• Molecular Weight: 35 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

COAB; PPC synthetase; PPCS

Quick Database Links

Gene Symbol

Phosphopantothenate--cysteine ligase

UniProt

Q9HAB8