PPAN Antibody

• Catalog Number: orb412594
• Category: Antibodies
• Description: The PPAN Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human PPAN .Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human PPAN
• UniProt ID: Q9NQ55, Q91YU8
• Tested applications: IF, IHC, WB
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: BXDC3; SSF1; Suppressor of SWI4 1 homolog; Ssf-1;
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 56342, 235036

Western blot analysis of PPAN expression in HepG2 (A), mouse heart (B) whole cell lysates. (Predicted band size: 51; 53; 58 kD; Observed band size: 53; 55 kD)

Immunofluorescent analysis of PPAN staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.