Pontin 52 Antibody

• Catalog Number: orb340959
• Category: Antibodies
• Description: The Pontin 52 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human Pontin 52 .Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human Pontin 52
• UniProt ID: P60123, P60122, Q9Y265
• Tested applications: IF, IHC, WB
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:50-1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: INO80H; NMP238; TIP49; TIP49A; RuvB-like 1; 49 kDa
• Research Area: Cancer, DNA Damage, Epigenetics
• Note: For research use only
• Entrez: 65137, 56505, 8607

Western blot analysis of Pontin 52 expression in MCF7 (A), HepG2 (B), mouse spleen (C), mouse brain (D) whole cell lysates. (Predicted band size: 42; 50 kD; Observed band size: 55 kD)

Immunohistochemical analysis of Pontin 52 staining in human gastric cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Pontin 52 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.