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Anti-Poliovirus Receptor/PVR Antibody

Catalog Number: orb865722

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb865722
CategoryAntibodies
DescriptionAnti-Poliovirus Receptor/PVR Antibody. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, ICC, IF, IHC, WB
ReactivityHuman
IsotypeRabbit IgG
ImmunogenE.coli-derived human Poliovirus Receptor/PVR recombinant protein (Position: D28-R417).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW70 kDa
UniProt IDP15151
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesNKG2-D type II integral membrane protein; Killer c
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NoteFor research use only
Application notesWestern blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
Expiration Date12 months from date of receipt.
Anti-Poliovirus Receptor/PVR Antibody

IF analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody. Poliovirus Receptor/PVR was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-Poliovirus Receptor/PVR Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-Poliovirus Receptor/PVR Antibody

IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody. Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Poliovirus Receptor/PVR Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-Poliovirus Receptor/PVR Antibody

Western blot analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HT1080 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Poliovirus Receptor/PVR antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Poliovirus Receptor/PVR at approximately 70 kDa. The expected band size for Poliovirus Receptor/PVR is at 45 kDa.

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    FC,  IHC,  WB

    Human, Mouse

    Mouse

    Monoclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-Poliovirus Receptor/PVR Antibody (monoclonal, 9B9F1) [orb1145773]

    IHC,  WB

    Human, Mouse

    Mouse

    Monoclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-Poliovirus Receptor/PVR Antibody [orb738463]

    ELISA,  FC,  ICC,  IF,  IHC,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-Poliovirus Receptor/PVR Antibody (monoclonal, 3B11E9) [orb1145771]

    FC,  IHC,  WB

    Human, Mouse

    Mouse

    Monoclonal

    Unconjugated

    100 μg, 10 μg
  • Anti-Poliovirus Receptor/PVR Antibody [orb745914]

    ELISA,  WB

    Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg