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Anti-Poliovirus Receptor/PVR Antibody

Catalog Number: orb745914

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb745914
CategoryAntibodies
DescriptionAnti-Poliovirus Receptor/PVR Antibody. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, WB
ReactivityMouse, Rat
IsotypeRabbit IgG
ImmunogenE.coli-derived mouse Poliovirus Receptor/PVR recombinant protein (Position: R24-R345).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW60-80 kDa
UniProt IDQ91WP1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesNKG2-D type II integral membrane protein; Killer c
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NoteFor research use only
Application notesWestern blot, 0.25-0.5μg/ml, Mouse, Rat ELISA, 0.1-0.5μg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-Poliovirus Receptor/PVR Antibody

Western blot analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Poliovirus Receptor/PVR antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Poliovirus Receptor/PVR at approximately 60-80 KD. The expected band size for Poliovirus Receptor/PVR is at 60-80 KD.

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    FC,  IHC,  WB

    Human, Mouse

    Mouse

    Monoclonal

    Unconjugated

    10 μg, 100 μg
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    Human, Mouse

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    ELISA,  FC,  ICC,  IF,  IHC,  WB

    Human

    Rabbit

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    10 μg, 100 μg
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    FC,  IHC,  WB

    Human, Mouse

    Mouse

    Monoclonal

    Unconjugated

    100 μg, 10 μg
  • Anti-Poliovirus Receptor/PVR Antibody [orb865722]

    ELISA,  ICC,  IF,  IHC,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg