Anti-PLXDC2 (4G3) rabbit mAb Biotin Conjugate

• Catalog Number: orb1945972
• Category: Antibodies
• Description: Anti-PLXDC2 (4G3) rabbit mAb Biotin Conjugate
• Clonality: Monoclonal
• Isotype: Rabbit IgGk
• Conjugation: Biotin
• Reactivity: Human
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: 1X PBS, 0.05% (w/v) Proclin300
• Purification: Ion Exchange
• Immunogen: HEK293 cells expressing human PLXDC2
• UniProt ID: Q6UX71
• Tested applications: WB
• Dilution range: ELISA 1:5000-10000 Western blotting 1:1000-2000 ICH 1:1000-2000
• Cross Reactivity: Predicted to work with mouse
• Clone Number: 4G3
• Storage: 2-8°C
• Alternative names: TEM7R, 1200007L24Rik, 5430431D22Rik
• Note: For research use only

Microtiter wells were coated with human PLXDC2-ECD (extracellular domain, truncated a transmembrane domain from PLXDC2) at 5 ug/mL. Anti-PLXDC2 (4G3) rabbit mAb Biotin Conjugate (Cat#orb1945972) was diluted at 1 ug/mL and 0.1 ug/mL. Anti-PLXDC2 (4G3) rabbit mAb Biotin Conjugate show very specific binding to human PLXDC2-ECD antigen.

A sandwich ELISA was performed using anti-PLXDC2 specific rabbit mAb: 3B8 (Cat#XXXX) as a capture antibody and 4G3-biotin (Cat#orb1945972) as a detection antibody. Human PLXDC2-ECD was serially diluted 1:1 starting at 5 ug/mL. Anti-PLXDC2 rabbit mAb 3B8 and 4G3-biotin detected hPLXDC2-ECD antigen at very high sensitivity as low as 100 pg/mL.

Western blot analysis of extracts from HEK293 cells, untransfected or transfected with human PLXDC2 gene, with anti-PLXDC2 (4G3) rabbit mAb Biotin Conjugate at 0.5 μg/mL Cat. # orb1945972