Anti-PKC delta/PRKCD Antibody

• Catalog Number: orb234285
• Category: Antibodies
• Description: Anti-PKC delta/PRKCD Antibody. Tested in IF, ICC, WB applications. This antibody reacts with Human.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human PKC delta recombinant protein (Position: M1-E160). Human PKC delta shares 95% and 93% amino acid (aa) sequence identity with mouse and rat PKC delta, respctively.
• Antibody Type: Primary Antibody
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 77 kDa
• UniProt ID: Q05655
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Protein kinase C delta type; 2.7.11.13; Tyrosine-p
• Note: For research use only
• Application notes: Western blot, 0.1-0.5μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Expiration Date: 12 months from date of receipt.

IF analysis of PKC delta using anti-PKC delta antibody. PKC delta was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 µg/mL rabbit anti-PKC delta Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-PKC delta antibody, Western blotting. All lanes: Anti PKC delta at 0.5 ug/ml. Lane 1: HELA Whole Cell Lysate at 40 ug. Lane 2: SMMC Whole Cell Lysate at 40 ug. Lane 3: U87 Whole Cell Lysate at 40 ug. Lane 4: JURKAT Whole Cell Lysate at 40 ug. Lane 5: CEM Whole Cell Lysate at 40 ug. Lane 6: A549 Whole Cell Lysate at 40 ug. Predicted bind size: 77 KD. Observed bind size: 77 KD.