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PKC beta 1/PRKCB Rabbit Polyclonal Antibody

SKU: orb234365

Description

The PKC beta 1/PRKCB Rabbit Polyclonal Antibody is an unconjugated antibody raised in rabbit. It is immunogen affinity purified and suitable for use in FC, IP, and WB applications. This antibody targets the Protein kinase C beta type in Human, Mouse, and Rat samples.

Research Area

Cell Biology, Epigenetics & Chromatin, Protein Biochemistry, Signal Transduction

Images & Validation

Tested ApplicationsFC, IP, WB
Dilution RangeWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunoprecipitation, 0.5-2 μg/ml, Human Flow Cytometry(Fixed), 1-3 μg/1x10^6 cells, Human
ReactivityHuman, Mouse, Rat
Application Notes
For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenE.coli-derived human PKC beta 1 recombinant protein (Position: E542-V671). Human PKC beta 1 shares 100% amino acid (aa) sequence identity with both mouse and rat PKC beta 1.
TargetProtein kinase C beta type
Molecular Weight80 kDa
PurificationImmunogen affinity purified.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Protein kinase C beta type; PKC-B; PKC-beta; 2.7.11.13; PRKCB; PKCB, PRKCB1

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UniProt Details

No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
IP
Immunoprecipitation
View Protocol

Available Sizes

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100 μg
$ 470.00
Free Secondary Antibody (20 ul)0/0

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