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Anti-PITRM1 Antibody

Catalog Number: orb865446

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb865446
CategoryAntibodies
DescriptionAnti-PITRM1 Antibody. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, FC, ICC, IF, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenE.coli-derived human PITRM1 recombinant protein (Position: A33-K1037).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW117 kDa
UniProt IDQ5JRX3
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesImmunoglobulin lambda-like polypeptide 1; CD179 an
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NoteFor research use only
Application notesWestern blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
Expiration Date12 months from date of receipt.
Anti-PITRM1 Antibody

Flow Cytometry analysis of U937 cells using anti-PITRM1 antibody. Overlay histogram showing U937 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PITRM1 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-PITRM1 Antibody

IF analysis of PITRM1 using anti-PITRM1 antibody. PITRM1 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-PITRM1 Antibody overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-PITRM1 Antibody

Western blot analysis of PITRM1 using anti-PITRM1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PITRM1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PITRM1 at approximately 117 kDa. The expected band size for PITRM1 is at 117 kDa.

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    Unconjugated

    200 μl, 100 μl, 50 μl
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    Polyclonal

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    ELISA,  FC,  ICC,  IF,  WB

    Human, Mouse, Rat

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    Polyclonal

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    100 μg
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    ELISA,  FC,  ICC,  IF,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

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    100 μg