PIGR Rabbit Polyclonal Antibody

SKU: orb334508

Description

Anti-PIGR Antibody. Tested in WB applications. This antibody reacts with Human.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human Flow Cytometry(Fixed), 1-3 μg/1x10^6 cells, Human
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PIGR, different from the related rat sequence by eighteen amino acids.
• Target: Polymeric immunoglobulin receptor
• Molecular Weight: 83 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Polymeric immunoglobulin receptor; PIgR; Poly-Ig receptor; Hepatocellular carcinoma-associated protein TB6; Secretory component; PIGR

Western blot analysis of PIGR using anti-PIGR antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: SKOV Whole Cell Lysate, Lane 3: MCF-7 Whole Cell Lysate, Lane 4: SW620 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIGR antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PIGR at approximately 83 kDa. The expected band size for PIGR is at 83 kDa.

Quick Database Links

Gene Symbol

Polymeric immunoglobulin receptor

UniProt

P01833