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PHD2/EGLN1 Rabbit Polyclonal Antibody

SKU: orb2803668

Description

PHD2/EGLN1 Rabbit Polyclonal Antibody

Research Area

Metabolism Research

Images & Validation

Tested ApplicationsFC, ICC, IP, WB
Dilution RangeWestern blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Immunoprecipitation, 0.5-2 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ReactivityHuman, Mouse, Rat
Application Notes
For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenA synthetic peptide corresponding to a sequence at the C-terminus of human PHD2/EGLN1. Human PHD2/EGLN1 shares 100% amino acid (aa) sequence identity with both mouse and rat PHD2/EGLN1.
TargetEgl nine homolog 1
Molecular Weight46 kDa
PurificationImmunogen affinity purified.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

C1orf12; ECYT3; Egl nine homolog 1; EGLN1; HIF PH2; HIF prolyl hydroxylase 2; HIFPH2; HPH 2; HPH2; PHD2; PHD2/EGLN1; PNAS 137; SM 20; SM20; ZMYND6

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UniProt Details

No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
ICC
Immunocytochemistry
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IP
Immunoprecipitation
View Protocol

Available Sizes

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100 μg
$ 470.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2-4 weeks
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