Peroxiredoxin 5/PRDX5 Antibody

• Catalog Number: orb251567
• Category: Antibodies
• Description: Peroxiredoxin 5/PRDX5 Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Purification: Immunogen affinity purified.
• Immunogen: E.coli-derived human Peroxiredoxin 5 recombinant protein (Position: E66-D198). Human Peroxiredoxin 5 shares 91% amino acid (aa) sequence identity with both mouse and rat Peroxiredoxin 5.
• UniProt ID: P30044
• MW: 22 kDa
• Tested applications: FC, ICC, IF, IHC, WB
• Application notes: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Cross Reactivity: No cross-reactivity with other proteins.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Peroxiredoxin-5, mitochondrial; 1.11.1.15; Alu cor
• Note: For research use only

Anti-Peroxiredoxin 5 Picoband antibody, IHC(P): Human Lung Cancer Tissue.

Anti-Peroxiredoxin 5 Picoband antibody, IHC(P): Mouse Intestine Tissue.

Anti-Peroxiredoxin 5 Picoband antibody, IHC(P): Rat Intestine Tissue.

Anti-Peroxiredoxin 5 Picoband antibody, Western blotting. All lanes: Anti Peroxiredoxin 5 at 0.5 ug/ml. Lane 1: A549 Whole Cell Lysate at 40 ug. Lane 2: Rat Brain Tissue Lysate at 50 ug. Lane 3: Mouse Brain Tissue Lysate at 50 ug. Predicted bind size: 22 KD. Observed bind size: 22 KD.

Flow Cytometry analysis of A549 cells using anti-Peroxiredoxin 5 antibody. Overlay histogram showing A549 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Peroxiredoxin 5 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

IF analysis of Peroxiredoxin 5 using anti-Peroxiredoxin 5 antibody. Peroxiredoxin 5 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 µg/mL rabbit anti-Peroxiredoxin 5 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IHC analysis of Peroxiredoxin 5 using anti-Peroxiredoxin 5 antibody. Peroxiredoxin 5 was detected in immunocytochemical section of SMMC Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 µg/ml rabbit anti-Peroxiredoxin 5 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.