Anti-Peroxiredoxin 1 Antibody

• Catalog Number: orb1421497
• Category: Antibodies
• Description: Mouse monoclonal antibody to Peroxiredoxin 1.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PRDX1
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: WB: WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200), IF: WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200)
• Conjugation: Unconjugated
• UniProt ID: Q06830
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: PAGA; PAGB; TDPX2; Peroxiredoxin-1; Natural killer
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Peroxiredoxin 1 expression in MCF7 (A), mouse brain (B), rat kidney (C) whole cell lysates.

Immunofluorescent analysis of Peroxiredoxin 1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).