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Anti-Pea3/ETV4 Antibody
Catalog Number: orb316562
| Catalog Number | orb316562 |
|---|---|
| Category | Antibodies |
| Description | Anti-Pea3/ETV4 Antibody. Tested in WB applications. This antibody reacts with Human, Rat. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | WB |
| Reactivity | Human, Rat |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Pea3, different from the related mouse sequence by seven amino acids. |
| Antibody Type | Primary Antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 68 kDa |
| UniProt ID | P43268 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | ETS translocation variant 4; Adenovirus E1A enhanc Read more... |
| Note | For research use only |
| Application notes | Western blot, 0.1-0.5μg/ml, Human, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of Pea3 using anti-Pea3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Lung Tissue Lysate at 50 ug, Lane 2: HELA Whole Cell Lysate at 40 ug, Lane 3: A549 Whole Cell Lysate at 40 ug, Lane 4: SW620 Whole Cell Lysate at 40 ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pea3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Pea3 at approximately 68 kDa. The expected band size for Pea3 is at 54 kDa.
