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Anti-PDGF AA/PDGFA Antibody

Catalog Number: orb865711

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb865711
CategoryAntibodies
DescriptionAnti-PDGF AA/PDGFA Antibody. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, ICC, IF, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenE.coli-derived human PDGF AA/PDGFA recombinant protein (Position: S87-T211).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW24 kDa
UniProt IDP04085
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesMitochondrial uncoupling protein 2; UCP 2; Solute
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NoteFor research use only
Application notesWestern blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
Expiration Date12 months from date of receipt.
Anti-PDGF AA/PDGFA Antibody

IF analysis of PDGF AA/PDGFA using anti-PDGF AA/PDGFA antibody. PDGF AA/PDGFA was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-PDGF AA/PDGFA Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-PDGF AA/PDGFA Antibody

Western blot analysis of PDGF AA/PDGFA using anti-PDGF AA/PDGFA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGF AA/PDGFA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDGF AA/PDGFA at approximately 24 kDa. The expected band size for PDGF AA/PDGFA is at 24 kDa.

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    ELISA,  ICC,  IF,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    iFluor647

    100 μg
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    ELISA,  ICC,  IF,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

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    100 μg
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    ELISA,  ICC,  IF,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

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    100 μg
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    ELISA,  ICC,  IF,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    HRP

    100 μg
  • Anti-PDGF AA/PDGFA Antibody [orb2597878]

    ELISA,  ICC,  IF,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    FITC

    100 μg