PD-L1/Cd274 Antibody

SKU: orb745905

Description

Anti-PD-L1/Cd274 Antibody. Tested in ELISA, WB applications. This antibody reacts with Mouse.

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Mouse
• Application Notes:
  Western blot, 0.25-0.5μg/ml, Mouse ELISA, 0.1-0.5μg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived mouse PD-L1/Cd274 recombinant protein (Position: E45-H239).
• Molecular Weight: 40 kDa
• Purification: Immunogen affinity purified.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Disclaimer: For research use only

Alternative Names

Programmed cell death 1 ligand 1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7 homolog 1; B7-H1; CD274; CD274; B7H1, PDCD1L1, PDCD1LG1, PDL1

Western blot analysis of PD-L1/Cd274 using anti-PD-L1/Cd274 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD-L1/Cd274 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PD-L1/Cd274 at approximately 40 KD. The expected band size for PD-L1/Cd274 is at 40 KD.

Quick Database Links

UniProt

Q9EP73