PCSK9 Rabbit Polyclonal Antibody

SKU: orb745916

Description

Anti-PCSK9 Antibody. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Research Area

Cancer Biology, Cardiovascular Research, Hematology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: Western blot, 0.25-0.5μg/ml, Mouse, Rat ELISA, 0.1-0.5μg/ml, -
• Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived mouse PCSK9 recombinant protein (Position: Q182-H560).
• Target: Proprotein convertase subtilisin/kexin type 9
• Molecular Weight: 74 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FH3; HCHOLA3; LDLCQ1; NARC 1; NARC1; PC9; PCSK9; Proprotein convertase 9

Western blot analysis of PCSK9/Pcsk9 using anti-PCSK9/Pcsk9 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK9/Pcsk9 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PCSK9/Pcsk9 at approximately 74 KD. The expected band size for PCSK9/Pcsk9 is at 74 KD.

Quick Database Links

Gene Symbol

Proprotein convertase subtilisin/kexin type 9

UniProt

Q80W65