P2RX4 Rabbit Polyclonal Antibody

SKU: orb234350

Description

Anti-P2RX4 Antibody. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Research Area

Cardiovascular Research, Cell Biology, Neuroscience

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat, Monkey
• Reactivity: Human, Monkey, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human P2RX4 recombinant protein (Position: N262-Q388). Human P2RX4 shares 91% and 90% amino acid (aa) sequence identity with mouse and rat P2RX4, respectively.
• Target: P2X purinoceptor 4
• Molecular Weight: 72 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

P2X purinoceptor 4; P2X4; ATP receptor; Purinergic receptor; P2RX4

Western blot analysis of P2RX4 using anti-P2RX4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A375 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: rat NRK whole cell lysates, Lane 8: rat RH-35 whole cell lysates, Lane 9: mouse kidney tissue lysates, Lane 10: mouse liver tissue lysates, Lane 11: mouse Raw264.7 whole cell lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2RX4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for P2RX4 at approximately 72 KD. The expected band size for P2RX4 is at 43 KD.

Quick Database Links

Gene Symbol

P2X purinoceptor 4

UniProt

Q99571