Anti-Osteocalcin/BGLAP Antibody

• Catalog Number: orb371654
• Category: Antibodies
• Description: Anti-Osteocalcin/BGLAP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC
• Reactivity: Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived rat Osteocalcin recombinant protein (Position: Y50-V99). Rat Osteocalcin shares 80.9% and 68.9% amino acid (aa) sequence identity with human and mouse Osteocalcin, respectively.
• Antibody Type: Primary Antibody
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 29 kDa, 60 kDa
• UniProt ID: P04640
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Osteocalcin; Bone Gla protein; BGP; Gamma-carboxyg
• Note: For research use only
• Application notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat Flow Cytometry (Fixed), 1-3μg/1x106 cells, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of L6 cells using anti-Osteocalcin antibody. Overlay histogram showing L6 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Osteocalcin Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

IHC analysis of Osteocalcin using anti-Osteocalcin antibody. Osteocalcin was detected in paraffin-embedded section of rat tibia tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Osteocalcin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.