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Anti-Osteocalcin/BGLAP Antibody
Catalog Number: orb371652
| Catalog Number | orb371652 |
|---|---|
| Category | Antibodies |
| Description | Anti-Osteocalcin/BGLAP Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Immunogen | E. coli-derived human Osteocalcin recombinant protein (Position: Y52-V100). Human Osteocalcin shares 69% and 80.9% amino acid (aa) sequence identity with mouse and rat Osteocalcin, respectively. |
| Antibody Type | Primary Antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 29 kDa, 60 kDa |
| UniProt ID | P02818 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Osteocalcin; Bone Gla protein; BGP; Gamma-carboxyg Read more... |
| Note | For research use only |
| Application notes | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
| Expiration Date | 12 months from date of receipt. |
IHC analysis of Osteocalcin using anti-Osteocalcin antibody. Osteocalcin was detected in a paraffin-embedded section of human osteosarcoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Osteocalcin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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