Anti-Occludin/OCLN Antibody

• Catalog Number: orb234284
• Category: Antibodies
• Description: Anti-Occludin/OCLN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Occludin recombinant protein (Position: N373-T522). Human Occludin shares 89% amino acid (aa) sequence identity with both mouse and rat Occludin.
• Antibody Type: Primary Antibody
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 59 kDa
• UniProt ID: Q16625
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Occludin; OCLN
• Note: For research use only
• Application notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, HumanWestern blot, 0.1-0.5μg/ml, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Expiration Date: 12 months from date of receipt.

Anti-Occludin antibody, Western blotting. All lanes: Anti Occludin at 0.5 ug/ml. Lane 1: SW620 Whole Cell Lysate at 40 ug. Lane 2: COLO320 Whole Cell Lysate at 40 ug. Lane 3: A549 Whole Cell Lysate at 40 ug. Lane 4: 293T Whole Cell Lysate at 40 ug Lane 5: U87 Whole Cell Lysate at 40 ug. Predicted bind size: 59 KD. Observed bind size: 59 KD.

Anti-Occludin antibody, IHC(P): Human Mammary Cancer Tissue.