NT-4/NTF4/NT Rabbit Polyclonal Antibody

SKU: orb669194

Description

Anti-NT-4/NTF4 Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human NT-4/NTF4, which shares 83.3% amino acid (aa) sequence identity with both mouse and rat NT-4/NTF4.
• Target: Neurotrophin-4
• Molecular Weight: 24 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

neurotrophin 4; Neurotrophin 5; Neutrophic factor 4; NT 4; NT 4/5; NT 5; NT4; NT5; NTF4; NTF5

Western blot analysis of NT-4/NTF4 using anti-NT-4/NTF4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat RH-35 whole cell lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NT-4/NTF4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NT-4/NTF4 at approximately 24 KD. The expected band size for NT-4/NTF4 is at 24 KD.

Quick Database Links

Gene Symbol

Neurotrophin-4

UniProt

P34130