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Catalog Number | orb1743777 |
---|---|
Category | Antibodies |
Description | Anti-NR2C2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IHC, WB |
Reactivity | Human, Monkey, Mouse, Rat |
Immunogen | E.coli-derived human NR2C2 recombinant protein (Position: R7-L596). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat, Monkey Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 68 kDa |
UniProt ID | P49116 |
Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of 293T cells using anti-NR2C2 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis using anti-NR2C2 antibody.1:human HeLa cell;2:human Jurkat cell;3:human PC-3 cell;4:human 293T cell;5:monkey COS-7 cell;6:human HEL cell;7:human U20S cell;8:human SH-SY5Y cell;9:rat C6 cell;10:mouse thymus tissue;11:mouse NIH/3T3 cell.
IHC analysis of NR2C2 using anti-NR2C2 antibody. NR2C2 was detected in a paraffin-embedded section of human acinic cell carcinoma parotid tissue.
IHC analysis of NR2C2 using anti-NR2C2 antibody. NR2C2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue.
WB | |
Human, Porcine | |
Rabbit | |
Polyclonal | |
Unconjugated |
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