Anti-NOTCH2 Antibody

• Catalog Number: orb214327
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NOTCH2
• Target: NOTCH2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NOTCH2. The exact sequence is proprietary.
• UniProt ID: Q9QW30, Q04721
• Tested applications: IH, WB
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-hN2 antibody, anti-Motch B antibody, anti-N2E
• Note: For research use only
• Entrez: 4853, 29492

Western blot analysis of NOTCH2 expression in HEK293T (A), HepG2 (B) whole cell lysates. (Predicted band size: 265 kD; Observed band size: 110 kD)

Immunohistochemical analysis of NOTCH2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.