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Anti-nNOS (neuronal)/NOS1 Antibody

Catalog Number: orb389501

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb389501
CategoryAntibodies
DescriptionAnti-nNOS (neuronal)/NOS1 Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
ClonalityPolyclonal
Species/HostRabbit
IsotypeRabbit IgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLyophilized
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
ImmunogenA synthetic peptide corresponding to a sequence at the C-terminus of human nNOS(neuronal), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
UniProt IDP29475
MW160 kDa
Tested applicationsWB
Application notesWestern blot, 0.1-0.5μg/ml, Mouse, Rat, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesNitric oxide synthase, brain; 1.14.13.39; Constitu
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NoteFor research use only
Anti-nNOS (neuronal)/NOS1 Antibody

Western blot analysis of nNOS using anti-nNOS antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. lane 1: rat brain tissue lysates, lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-nNOS antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for nNOS at approximately 160KD. The expected band size for nNOS is at 160KD.

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