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Anti-NFAT2/NFATC1 Antibody

Catalog Number: orb570343

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb570343
CategoryAntibodies
DescriptionAnti-NFAT2/NFATC1 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenE.coli-derived human NFAT2/NFATC1 recombinant protein (Position: F374-E710).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW120 kDa
UniProt IDO95644
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesNuclear factor of activated T-cells, cytoplasmic 1
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NoteFor research use only
Application notesWestern blot, 0.5-1μg/ml ELISA, 0.1-0.5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-NFAT2/NFATC1 Antibody

Western blot analysis of NFAT2/NFATC1 using anti-NFAT2/NFATC1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT2/NFATC1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFAT2/NFATC1 at approximately 120KD. The expected band size for NFAT2/NFATC1 is at 101KD.

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    ELISA,  IHC,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 10 μg
  • Anti-NFAT2 NFATC1 Rabbit Monoclonal Antibody [orb547257]

    FC,  ICC,  IF,  WB

    Human

    Rabbit

    Monoclonal

    Unconjugated

  • Anti-NFAT2/NFATC1 Antibody [orb2608843]

    ELISA,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    iFluor647

    100 μg
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    ELISA,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    PE

    100 μg
  • Anti-NFAT2/NFATC1 Antibody [orb2608845]

    ELISA,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    APC

    100 μg