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NDUFA1 Rabbit Polyclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | FC, ICC, IF, IHC, IHC-Fr, WB |
|---|---|
| Dilution Range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry(Fixed), 1-3 μg/1x10^6 cells, Human |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human NDUFA1, different from the related rat and mouse sequences by one amino acid. |
| Target | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 |
| Molecular Weight | 8 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot analysis of Lane 1: Rat Cardiac Muscle Tissue Lysate, Lane 2: Rat Liver Tissue Lysate, Lane 3: Rat Kidney Tissue Lysate, Lane 4: Rat Brain Tissue Lysate, Lane 5: HT1080 Cell Lysate using NDUFA1 antibody

Immunohistochemical analysis of formali

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NDUFA1 Rabbit Polyclonal Antibody (orb76175)
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