NCX1/SLC8A1 Rabbit Polyclonal Antibody

SKU: orb1743822

Description

Anti-NCX1/SLC8A1 Antibody. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Research Area

Cell Biology, Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Dilution Range: Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human ELISA, 0.1-0.5 μg/ml, -
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human NCX1/SLC8A1 recombinant protein (Position: E301-Q686).
• Target: Sodium/calcium exchanger 1
• Molecular Weight: 120 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CNC; DKFZp779F0871; FLJ37694; FLJ43417; NCX1; SLC8A1; Sodium/calcium exchanger 1

IF analysis of SLC8A1 using anti-SLC8A1 antibody. SLC8A1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-SLC8A1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The tissue section was developed using Phalloidin-iFluor 594 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Western blot analysis of NCX1/SLC8A1 using anti-NCX1/SLC8A1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCX1/SLC8A1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NCX1/SLC8A1 at approximately 120 kDa. The expected band size for NCX1/SLC8A1 is at 120 kDa.

Quick Database Links

Gene Symbol

Sodium/calcium exchanger 1

UniProt

P32418