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Catalog Number | orb1786089 |
---|---|
Category | Antibodies |
Description | Anti-NAP1L1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | A synthetic peptide corresponding to a sequence of human NAP1L1 (CKLKARQLTVQMMQNPQILAALQE). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 55 kDa |
UniProt ID | P55209 |
Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of PC-3 cells using anti-NAP1L1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of NAP1L1 using anti-NAP1L1 antibody.Lane 1:human HeLa cell;2:human 293T cell;3:human Jurkat cell;4:rat thymus tissue;5:rat PC-12 cell;6:mouse thymus tissue;7:mouse NIH/3T3 cell.
IF analysis of NAP1L1 using anti-NAP1L1 antibody. NAP1L1 was detected in an immunocytochemical section of U2OS cells.
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