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Catalog Number | orb1743965 |
---|---|
Category | Antibodies |
Description | Anti-NANP Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IHC, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | E.coli-derived human NANP recombinant protein (Position: R5-M246). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 30 kDa |
UniProt ID | Q8TBE9 |
Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of HepG2 cells using anti-NANP antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of NANP using anti-NANP antibody.Lane 1:human SH-SY5Y cell;2:human HepG2 cell;3:human A431 cell;4:human Jurkat cell;5:rat liver tissue;6:rat RH35 cell;7:mouse liver tissue;8:mouse NIH/3T3 cell.
IHC analysis of NANP using anti-NANP antibody. NANP was detected in a paraffin-embedded section of human liver cancer tissue.
IHC analysis of NANP using anti-NANP antibody. NANP was detected in a paraffin-embedded section of human lung adenocarcinoma tissue.
IHC analysis of NANP using anti-NANP antibody. NANP was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue.
IHC analysis of NANP using anti-NANP antibody. NANP was detected in a paraffin-embedded section of human testicular seminoma tissue.
IHC analysis of NANP using anti-NANP antibody. NANP was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue.
IHC analysis of NANP using anti-NANP antibody. NANP was detected in a paraffin-embedded section of human tonsil tissue.
IHC analysis of NANP using anti-NANP antibody. NANP was detected in a paraffin-embedded section of rat brain tissue.
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