MURF1/TRIM63 Rabbit Polyclonal Antibody

SKU: orb669208

Description

Anti-MURF1/TRIM63 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Monkey.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: Western blot, 0.25-0.5μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat ELISA, 0.1-0.5μg/ml, -
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human MURF1/TRIM63 recombinant protein (Position: D2-E329).
• Target: E3 ubiquitin-protein ligase TRIM63
• Molecular Weight: 40 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: 500 µg/ml
• Disclaimer: For research use only

Alternative Names

IRF; Iris RING finger protein; MuRF 1; MURF1; MURF2; RING finger protein 28; RNF28; SMRZ; TRIM63; tripartite motif containing 63

Western blot analysis of MURF1/TRIM63 using anti-MURF1/TRIM63 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MURF1/TRIM63 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MURF1/TRIM63 at approximately 40 KD. The expected band size for MURF1/TRIM63 is at 40 KD.

Quick Database Links

Gene Symbol

E3 ubiquitin-protein ligase TRIM63

UniProt

Q969Q1