You have no items in your shopping cart.
Cart summary
Item 1 of 2
Item 1 of 2
Anti-MUC5B Antibody
Catalog Number: orb1145746
| Catalog Number | orb1145746 |
|---|---|
| Category | Antibodies |
| Description | Anti-MUC5B Antibody. Tested in ELISA, IHC applications. This antibody reacts with Human. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human MUC5B recombinant protein (Position: A5688-V5762). |
| Antibody Type | Primary Antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 70 kDa |
| UniProt ID | Q9HC84 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Folliculin; BHD skin lesion fibrofolliculoma prote Read more... |
| Note | For research use only |
| Application notes | Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
| Expiration Date | 12 months from date of receipt. |
IHC analysis of MUC5B using anti-MUC5B antibody. MUC5B was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-MUC5B Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of MUC5B using anti-MUC5B antibody. MUC5B was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-MUC5B Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
- Item 1 of 2
- Item 1 of 3
- Item 1 of 2
