mtTFA/Tfam Rabbit Polyclonal Antibody

SKU: orb745974

Description

Anti-mtTFA/Tfam Antibody. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Research Area

Cancer Biology, Epigenetics & Chromatin, Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: Western blot, 0.25-0.5μg/ml, Mouse, Rat ELISA, 0.1-0.5μg/ml, -
• Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived mouse mtTFA/Tfam recombinant protein (Position: M1-D211).
• Target: Transcription factor A, mitochondrial
• Molecular Weight: 23 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cell and organelle markers; Mitochondria Marker; Mitochondrial Marker; Mitochondrion Marker; MtTF1; mtTFA; TCF 6; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM; Transcription factor 6; Transcription factor 6 like 2

Western blot analysis of mtTFA/Tfam using anti-mtTFA/Tfam antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-mtTFA/Tfam antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for mtTFA/Tfam at approximately 23 KD. The expected band size for mtTFA/Tfam is at 23 KD.

Quick Database Links

Gene Symbol

Transcription factor A, mitochondrial

UniProt

P40630