MT-ND4/MT Rabbit Polyclonal Antibody

SKU: orb546348

Description

Anti-MT-ND4 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human.

Research Area

Disease Biomarkers

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml ELISA, 0.1-0.5μg/ml
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human MT-ND4 recombinant protein (Position: M10-S459).
• Target: NADH-ubiquinone oxidoreductase chain 4
• Molecular Weight: 36 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NADH-ubiquinone oxidoreductase chain 4; NADH dehydrogenase subunit 4; MT-ND4; MTND4; NADH4; ND4

Western blot analysis of MT-ND4 using anti-MT-ND4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates Lane 2: human HepG2 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human Caco-2 whole cell lysates Lane 5: human U2OS whole cell lysates Lane 6: human A549 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MT-ND4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MT-ND4 at approximately 36KD. The expected band size for MT-ND4 is at 52KD.

Quick Database Links

Gene Symbol

NADH-ubiquinone oxidoreductase chain 4

UniProt

P03905