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Catalog Number | orb1786102 |
---|---|
Category | Antibodies |
Description | Anti-MPHOSPH10 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, WB |
Reactivity | Human |
Immunogen | E.coli-derived human MPHOSPH10 recombinant protein (Position: L15-A661). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.25 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 120 kDa |
UniProt ID | O00566 |
Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of HepG2 cells using anti-MPHOSPH10 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of MPHOSPH10 using anti-MPHOSPH10 antibody.Lane 1:human HeLa cell;2:human HepG2 cell;3:human K562 cell;4:human HEL cell.
IF analysis of MPHOSPH10 using anti-MPHOSPH10 antibody and anti-Beta Tubulin antibody. MPHOSPH10 was detected in immunocytochemical section of HELA cell.
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