Cart summary

You have no items in your shopping cart.

Anti-MOCS1 Antibody

Catalog Number: orb1819373

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb1819373
CategoryAntibodies
DescriptionAnti-MOCS1 Antibody. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, ICC, IF, WB
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenE.coli-derived human MOCS1 recombinant protein (Position: E52-R584). Human MOCS1 shares 86.3% amino acid (aa) sequence identity with mouse MOCS1.
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW72 kDa
UniProt IDQ9NZB8
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative names70 kDa ribosomal protein S6 kinase 1 antibody, KS6
Read more...
NoteFor research use only
Application notesWestern blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
Expiration Date12 months from date of receipt.
Anti-MOCS1 Antibody

IF analysis of MOCS1 using anti-MOCS1 antibody. MOCS1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-MOCS1 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-MOCS1 Antibody

Western blot analysis of MOCS1 using anti-MOCS1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH-35 whole cell lysates, Lane 7: mouse liver whole cell lysates, Lane 8: mouse Hepa1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOCS1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MOCS1 at approximately 72 kDa. The expected band size for MOCS1 is at 70 kDa.

  • Anti-MOCS1 Antibody [orb1786106]

    ELISA,  FC,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-MOCS1 Antibody [orb1880991]

    IH,  WB

    Human, Mouse

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • Anti-MOCS1 Antibody [orb2584357]

    ELISA,  ICC,  IF,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    iFluor647

    100 μg
  • Anti-MOCS1 Antibody [orb2584358]

    ELISA,  ICC,  IF,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    PE

    100 μg
  • Anti-MOCS1 Antibody [orb2584359]

    ELISA,  ICC,  IF,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    APC

    100 μg