Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Rabbit Polyclonal Antibody

SKU: orb137872

Description

Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Rabbit Polyclonal Antibody

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat Western blot, 0.1-0.5μg/ml, Human, Rat
• Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.
• Target: Pyruvate dehydrogenase
• Molecular Weight: 85-91 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; 2.7.11.2; Pyruvate dehydrogenase kinase isoform 1; PDH kinase 1; PDK1; PDHK1

Western blot analysis of Lane 1: Rat Heart Tissue Lysate, Lane 2: Rat Skeletal Muscle Tissue Lysate, Lane 3: HELA Cell Lysate, Lane 4: M231 Cell Lysate, Lane 5: COLO320 Cell Lysate, Lane 6: SW620 Cell Lysate using PDK1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Human Prostatic Cancer Tissue using PDK1 antibody

Quick Database Links

Gene Symbol

Pyruvate dehydrogenase

UniProt

Q15118