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Anti-MIP-3 Beta/CCL19 Antibody
Catalog Number: orb1804657
| Catalog Number | orb1804657 |
|---|---|
| Category | Antibodies |
| Description | Anti-MIP-3 Beta/CCL19 Antibody. Tested in ELISA, IHC, Flow Cytometry applications. This antibody reacts with Human. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, FC, IHC |
| Reactivity | Human |
| Isotype | IgG |
| Immunogen | E.coli-derived human MIP-3 Beta/CCL19 recombinant protein (Position: G22-S98). |
| Antibody Type | Primary Antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 22 kDa |
| UniProt ID | Q99731 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | 70 kDa ribosomal protein S6 kinase 1 antibody, KS6 Read more... |
| Note | For research use only |
| Application notes | Immunohistochemistry, 2-5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
| Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of THP-1 cells using anti-MIP-3 Beta/CCL19 antibody. Overlay histogram showing THP-1 cells (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MIP-3 Beta/CCL19 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
IHC analysis of MIP-3 Beta/CCL19 using anti-MIP-3 Beta/CCL19 antibody. MIP-3 Beta/CCL19 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-MIP-3 Beta/CCL19 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
