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Anti-MIF4GD Antibody

Catalog Number: orb1879133

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb1879133
CategoryAntibodies
DescriptionRabbit polyclonal antibody to MIF4GD
ClonalityPolyclonal
Tested applicationsFC, IF, WB
ReactivityHuman, Mouse
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the Central region of human MIF4GD. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB (1/500 - 1/1000), IF/IC (1/10 - 1/50), FC (1/10 - 1/30)
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
Entrez69674, 57409
UniProt IDQ3UBZ5, A9UHW6
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesSLIP1; MIF4G domain-containing protein; SLBP-inter
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-MIF4GD Antibody

Western blot analysis of MIF4GD expression in mouse liver (A) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 25 kD)

Anti-MIF4GD Antibody

Immunofluorescent analysis of Anti-MIF4GD staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Anti-MIF4GD Antibody

Flow cytometric analysis of A431 cells using Anti-MIF4GD Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

  • MIF4GD Antibody [orb355627]

    ELISA

    Human

    Rabbit

    Polyclonal

    Unconjugated

    50 μg, 100 μg
  • MIF4GD Antibody, Biotin conjugated [orb355628]

    ELISA

    Human

    Rabbit

    Polyclonal

    Biotin

    50 μg, 100 μg
  • MIF4GD Antibody, FITC conjugated [orb355629]

    Human

    Rabbit

    Polyclonal

    FITC

    50 μg, 100 μg
  • MIF4GD Antibody, HRP conjugated [orb355630]

    ELISA

    Human

    Rabbit

    Polyclonal

    HRP

    50 μg, 100 μg