Anti-Mesothelin/MSLN Antibody

• Catalog Number: orb315179
• Category: Antibodies
• Description: Anti-Mesothelin/MSLN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC
• Reactivity: Mouse
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived mouse Mesothelin recombinant protein (Position: K308-L576). Mouse Mesothelin shares 58.4%% and 89.6% amino acid (aa) sequence identity with human and rat Mesothelin, respectively.
• Antibody Type: Primary Antibody
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 54 kDa
• UniProt ID: Q61468
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Mesothelin; Pre-pro-megakaryocyte-potentiating fac
• Note: For research use only
• Application notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse . Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Expiration Date: 12 months from date of receipt.

IHC analysis of Mesothelin using anti-Mesothelin antibody. Mesothelin was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Mesothelin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.